Composite

Part:BBa_I712064:Design

Designed by: Anja Korenčič   Group: iGEM07_Ljubljana   (2007-10-22)


pT7-IFNbeta


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PstI restriction site in interferon beta was mutated. Includes start and stop codon.


Source

Interferon beta comes from Origene human cDNA clone (NM_002176.2). T7 promoter comes from T7 baceriophage.

References